Western blot demonstrated that NT5E expression was significantly higher in gallbladder adenocarcinoma than in chronic cholecystitis (0.826 ± 0.011 vs 0.412 ± 0.017, P < 0.01), while the expression of FcGBP protein was significantly lower in gallbladder adenocarcinoma than in chronic cholecystitis (0.084 ± 0.017 vs 0.187 ± 0.025, P < 0.01). Here, FCGBP is linked to gallbladder adenocarcinoma.