Compared with the control group, both treated groups showed significantly higher levels of IL-12 (Figure 4A) and IFN-γ (Figure 4B), but significantly lower levels of immunosuppressive mediators IL-10 (Figure 4C) and TGF-β1 (Figure 4D) on day 5 post-treatment, a representative time-point for Tregs depletion and tumor growth inhibition. This evidence concerns the gene TGFB1 and neoplasm.