With the result of in vitro study, we demonstrated the possible correlation between inhibition of DJ-1 induced by hyperglycemia and ineffectiveness of postconditioning in diabetic rats and that PI3K/Akt signal pathway may be one of the major factors involved in the protective effect of DJ-1, which should be further elucidated by in vivo studies. Here, AKT1 is linked to Hyperglycemia.