The glioma cells transfected with the eEF-2 kinase-targeted siRNA showed a 70%–80% (LN229) and 85-90% (U251) reduction in eEF-2kinase protein expression at 60h after transfection, as compared with the cells transfected with a non-targeting siRNA (Figure 1C). The gene discussed is EEF2K; the disease is central nervous system cancer.