Immunohistochemistry staining showed that Beclin 1 was lowly expressed in 67.9% (72/106) ICC (Figure 1A) and 73.0% (54/74) ECC (Figure 1B) specimen (Figure 1C), especially in the tumor nest zone, whereas was moderately or strongly expressed in the normal adjacent tissues. The gene discussed is BECN1; the disease is intrahepatic cholangiocarcinoma.