Here, we found that the cholangiocarcinoma patients with lymph node metastasis (N1 stage) had a lower level of Beclin 1 than that of N0 stage patients (IHC score: 3.45±2.82 VS 4.60±2.97, Figure 2B). Here, BECN1 is linked to metastatic malignant neoplasm in the lymph nodes.