TNF and obesity due to melanocortin 4 receptor deficiency: In the rescue experiment, HFHSD supplemented with 20 μL of Kal-1 suppresses obesity and related pro-inflammatory responses like insulin response and blood glucose levels by reducing levels of IL-1α, IL-1β, IL-6, TNF-α, and MCP-1 and simultaneously elevating levels of anti-inflammatory IL-4 and IL-10 at week 15; however, here Kal-1 working amount was lower, that is, 5 μL (Figures 4(a) and 4(b)).