ESR1 and breast carcinoma: Since the GDS3283 is a breast cancer data set, we selected the ChIP-seq experiments from breast cancer cell lines (T47D and MCF7 cells) to generate the regulatory TF network with Q < 0.001 (Figure 2B), which contained five significant TFs: ESR1, GATA3, FOXA1, MYC and E2F1.