Hepatic steatosis was reversed in miR-33−/−Srebf1+/− mice compared with that in miR-33−/−Srebf1+/+ mice in both macro- and microscopic images and the liver triglyceride content of these mice was almost the same as that of miR-33+/+Srebf1+/+ and miR-33+/+Srebf1+/− mice (Supplementary Fig. S7b and Fig. 7e,f). This evidence concerns the gene SREBF1 and fatty liver disease.