Thus far the FISH method was mainly used on the one hand to check for certain chemotherapy targets such as EGFR [27] or HER-2 [28]; and on the other hand to define a set of four genetic markers (frequent copy number gains in chromosomes 1q32, 3q26, 5p15, and 8q24) applied to spiral CT-guided FNA cytology samples, which were highly sensitive for the diagnosis of lung cancer and highly specific in their ability to exclude cancer within a given specimen [29]. This evidence concerns the gene ERBB2 and cancer.