Analysis of both TFF3 (goblet cell marker) and CC10 (Clara cell marker) revealed a low level of expression at ALI day 0; however, a significant increase in expression was observed at ALI day 28 (TFF3, p < 0.03; CC10, p < 0.04), demonstrating the primary BC can differentiate into both the goblet and Clara cell types. This evidence concerns the gene TFF3 and breast cancer.