To analyze the function of the TGM3 protein in HNSCC cells, we transiently transfected mammalian expression vectors containing TGM3 into the WSU-HN4, HN13 and HN30 cell lines, all of which lack endogenous TGM3 expression (Figure 4A). The gene discussed is TGM3; the disease is head and neck squamous cell carcinoma.