Given their association with tumor invasion and metastasis, the purpose of this study was to examine HIF-2α, TWIST, and CXCR4 expression in PTC, to assess association of HIF-2α, TWIST, and CXCR4 expression with several clinicopathological indicators, and to evaluate potential usefulness of the three molecules in prediction for invasion and metastasis of PTC. Here, EPAS1 is linked to neoplasm.