Importantly, a similar induction of apoptosis was obtained in HL-60 AML cells transfected with a p53 expressing vector and treated with CX-4945, as judged by annexin V staining and FACS analysis and immunoblot analysis of p53 (which confirmed the good transfection efficiency) and of pro-caspase 3 levels (Figure 3G; n = 3, p < 0.05). Here, CASP3 is linked to acute myeloid leukemia.