To simulate the inflammatory conditions of MS lesions, we co-cultured pre-stimulated (IFN-γ and TNF-α) oligodendrocytes with CD3/CD28 pre-activated CD4+ T cells which were magnetic-activated cell sorting (MACS)-enriched for NKG2D (containing approx. 8–12% CD4+NKG2D+ T cells mimicking the human CSF data). Here, CD28 is linked to myeloid sarcoma.