To explore the effects of PD-associated mutations on the interaction of LRRK2 with Dnm1, Mfn1 and OPA1, we conducted co-IP experiments in HEK-293T cells expressing FLAG-tagged LRRK2 variants with either GFP-tagged Dnm1 or Myc-tagged Mfn1 and OPA1. The gene discussed is OPA1; the disease is Parkinson disease.