CCND1 and malignant colon neoplasm: In SW620 colon cancer cells treated with 2, 5, and 10 μM AZA197 for 24 h, Cyclin D1 protein expression decreased significantly by 16.8 ± 2.2% (2 μM), 18.6 ± 4.5% (5 μM) and 37.1 ± 14.1% (10 μM) (P < 0.05) compared to untreated controls as shown in Figure 5D. In HT-29 cells, Cyclin D1 protein expression was significantly reduced when treated with 5 μM (22 ± 5.5% reduction) and 10 μM (20.8 ± 9.9% reduction) (P < 0.049) but not with 2 μM AZA197 (Additional file 4: Figure S4D).