As with the primary mammary tumors, lung nodules expressed both polyoma middle T antigen and ERα (Figure 6A).There was no correlation between the number of lung metastases and survival time observed in the Sirt+/+ (r= 0.58, P=0.09), SirtY/+ (r= 0.36, P=0.30) or the SirtY/Y mice (r= 0.43, P=0.20)(Figure 6C). The gene discussed is ESR1; the disease is breast cancer.