To confirm the biological effects of TGFβ-induced phosphorylation of the PTEN C-terminus in lung cancer cells, we investigated whether mutation of phosphorylation sites in PTEN can affect both TGFβ-induced EMT and the migration ability of lung cancer cells by using a Dox-dependent gene expression system because several PTENWt reconstitution models have suggested that PTENWt transduction might induce a slow growth ratio in glioma cells [15]. The gene discussed is TGFB1; the disease is central nervous system cancer.