Figure 2A shows that maspinWT was predominantly localized in the cytoplasm. In contrast, maspinD346E was completely within the nuclear envelope as judged by the staining of lamin B (red). To confirm that the nuclear localization of maspinD346E was not cell line specific, lung cancer cell line H1299, which expresses no detectable level of maspin (Figure 2B), was infected by adenovirus. Similarly to the results with DU145 cells, maspinWT was localized in the cytoplasm, whereas maspinD346E was predominantly localized in the nuclei of H1299 cells (Figure 2C). This evidence concerns the gene SERPINB5 and lung carcinoma.