Western blot and real-time quantitative RT-PCR demonstrated that stable transfection of FOXD3 resulted in enhanced protein and transcript levels of FOXD3 and NDRG1 in NB cells, when compared to untransfected parental cells and those stably transfected with empty vector (mock) (Figure 2C and Figure 2D). The gene discussed is NDRG1; the disease is neuroblastoma.