To gain better insight into the role of HS3ST2 hypermethylation in non-small cell lung cancer (NSCLC), we characterized HS3ST2 hypermethylation in six lung cancer cell lines and investigated the effect of hypermethylation of HS3ST2 on the phenotype and prognosis of lung cancer in paraffin-embedded tissues from 298 primary non-small cell lung cancers (NSCLCs). This evidence concerns the gene HS3ST2 and non-small cell lung carcinoma.