Our results showed that oral cancer carcinogenesis overexpression of GAD1 occurs frequently and that it might be closely associated with invasion and metastasis of OSCC by β-catenin translocation and MMP7 activation, while further studies are needed to research the GAD1/β-catenin/MMP7 interaction, the current data indicated that GAD1 is likely a molecular marker for early detection of lymph node metastasis and an efficacious treatment target for preventing cancer metastasis in OSCCs. This evidence concerns the gene GAD1 and cancer.