Since the DUB activity of USP25 was involved in virus-induced type I IFN signaling, we further investigated whether the IFN antagonist function of USP25 is associated with the deubiquitination of RIG-I, IPS-1, TRAF2, and TRAF6, which are essential signaling components in the type I IFN pathway activated by virus infection. The gene discussed is USP25; the disease is viral infectious disease.