Since only bile salts bearing two negative charges, such as sulphated tauro- or glycoconjugated bile acids share Mrp2 transport with curcumin and sulphated BA conjugates are mostly present in cholestasis, it is difficult to explain the high liver and plasma concentration of bile acids after curcuma extract chronic feeding in mouse, by a competition of di-anionic conjugated bile acids and curcuma extract for the Mrp2 transport system [63,64]. The gene discussed is ABCC2; the disease is cholestasis.