To further validate that CD43 promotes cell transformation leading to tumor development, and considering that the signaling capacity of CD43 depends on its intracellular domain [19], we transfected a mutant form of CD43 lacking the intracellular domain in A549 cells expressing endogenous CD43 and tested whether this mutant would work as a dominant negative molecule, reducing wound healing and anchorage-independent cell growth capacities. Here, SPN is linked to neoplasm.