No relationship could be found between PA1-nuc protein expression with lymph node metastasis (P = 0.21), histopathology (P = 0.54), nuclear grade (P = 0.45), hormone receptor status (for ERα, P = 0.21; for PgR, P = 0.63), Her2 status (P = 0.10), and subtypes (P = 0.2). Here, NUCB1 is linked to metastatic malignant neoplasm in the lymph nodes.