Here, for the first time, we have provided evidence that diet-induced obesity reduces TSPO in WAT and BAT on the gene expression level in whole tissue (Figs. 1B and 2B), as well as on the protein expression level within mitochondrial extracts as demonstrated by ligand binding studies (Figs. 1D and 2D). Here, TSPO is linked to obesity due to melanocortin 4 receptor deficiency.