To further confirm the requirement of β3 for TGF-β1/H2O2/HOCl-mediated induction of metastatic phenotype, we treated HCC cells with TGF-β1/H2O2/HOCl in presence of CH50, a recombinant polypeptide which suppresses the function of αvβ3 [26]. Here, TGFB1 is linked to hepatocellular carcinoma.