We co-transfected osteosarcoma Saos-2 and HEK293 cells with the reporter plasmid together with FLAG-HIF-1α wt or with the C1772T mutant (corresponds to FLAG-HIF-1α/P582S) and the G1790A mutant (corresponds to FLAG-HIF-1α/A588T, respectively [20]). The gene discussed is HIF1A; the disease is osteosarcoma.