Similarly, conditioned media from prostate stromal cells isolated from aged EAF2−/− FVB/NJ mice induced a statistically significant increased growth rate in C4-2 prostate cancer cells compared to conditioned media from wild-type prostate stromal cells, suggesting that the altered stromal microenvironment induced by EAF2 loss in aged animals could stimulate epithelial cell growth through paracrine signaling (Fig. 5D). This evidence concerns the gene EAF2 and prostate carcinoma.