EAF2 and Familial prostate cancer: Similarly, conditioned media from prostate stromal cells isolated from aged EAF2−/− FVB/NJ mice induced a statistically significant increased growth rate in C4-2 prostate cancer cells compared to conditioned media from wild-type prostate stromal cells, suggesting that the altered stromal microenvironment induced by EAF2 loss in aged animals could stimulate epithelial cell growth through paracrine signaling (Fig. 5D).