In addition to high TB activity in the two-dose vaccination regimen, a single dose of Pfs25-CP VLPs plus Alhydrogel®, in the range from 0.2 μg to 25 μg of Pfs25, also elicited serum antibodies that completely blocked transmission in SMFA, and TB activity lasted up to 6 months post vaccination, further supporting the potential of Pfs25-CP VLPs as a TBV candidate. This evidence concerns the gene CP and tuberculosis.