To gain better insight into the role of HOXA11 gene in NSCLCs, we characterized the HOXA11 hypermethylation in vitro and further investigated the association between clinicopathological parameters and HOXA11 hypermethylation in paraffin-embedded tissues from 317 primary non-small cell lung cancers (NSCLCs). The gene discussed is HOXA11; the disease is non-small cell lung carcinoma.