HOXA11 and lung carcinoma: Dependence of the downregulation of HOXA11 in lung cancer cells on hypermethylation of the gene was validated by analyzing the re-expression and demethylation of silenced genes using RT-PCR (Fig. 2A), quantitative real-time PCR (Fig. 2B), MS-HRM assay (Fig. 2C), and EpiTYPERTM (Fig. 2D), after treatment of lung cancer cells with 10 μM 5-Aza-dC for 72 h.