Detailed analysis showed that the immunoreactivity of FZD3 protein, as shown by the ICC scores, had significant difference among CRC, CAD, colorectal polyp and normal colorectal epithelial tissues (Figure 2, P < 0.0001, Kruskal-Wallis test) and the range and median of the ICC scores in various specimen types were shown in Table 1. This evidence concerns the gene FZD3 and intrahepatic cholangiocarcinoma.