With the aim to investigate potential changes in cortical layering in isolated FCD cases from the frontal (FCx) and temporal (TCx) lobe and temporal FCD associated with AHS (FCD IIIa) we performed immunocytochemistry for NeuN, SMI32 and TLE4 and ISH for RORß and ER81 in serial sections of all cases destined for morphological analyses (Additional file 1: Table S1). This evidence concerns the gene RBFOX3 and mitochondrial DNA depletion syndrome 4a.