For all of the M. tuberculosis recombinant proteins tested, a significant higher number of IFN-γ producing effector T-cells were observed in active-TB patients compared to MTB-unexposed controls -Ag85B: controls 312 ± 363 (mean ± std) spots/million peripheral blood mononuclear cells (PBMCs), active-TB 2361 ± 1863; p < 0.0001; TB10.4: controls 184 ± 209, active-TB 2009 ± 1901; p < 0.0001; His-full2: controls 701 ± 859, active-TB 22183 ± 1173; p = 0.0381 - confirming that the cell mediated immune response is effectively triggered by the antigens produced during this study. The gene discussed is IFNG; the disease is tuberculosis.