I order to determine whether umbelliprenin induces apoptosis in CLL cells, cells were incubated with various concentrations of umbelliprenin in-vitro for 16, 20, 24 and 48 h and assayed for apoptosis with annexin V–FITC/PI double staining flowcytometry (Figure 2 and Table1). Here, ANXA5 is linked to B-cell chronic lymphocytic leukemia.