IGFBP1 and type 2 diabetes mellitus: Combining all six CpG sites together, the mean values of IGFBP1 DNA methylation levels were significantly increased in both newly diagnosed and treated patients in comparison with non-diabetic subjects (19.8%, 18.9% and 16.9%, P < 0.001 for both type 2 diabetes groups vs. controls) (Figure 1B).