Moreover a recent study from Baldus et al. evaluated K-Ras, BRAF and PI3K gene status into the primary tumor, comparing the tumor center and the invasion fronts showing that intratumoral heterogeneity of K-Ras, BRAF, and PIK3CA mutations was observed in 8%, 1%, and 5% of primary tumors, respectively [29]. Here, BRAF is linked to neoplasm.