While these latter studies utilized the prostate cancer cell lines, PC3 and PC3-AR, and immortalized normal prostate RWPE-1 cells as experimental models, it is well known that PC3 cells are AR-, p16INK4a- and p53-null [39], and RWPE-1 cells are p53- and Rb-null [41]. This evidence concerns the gene AR and Familial prostate cancer.