Although the hyh mutant mice are alive after the birth and die by 2 months of age [53], the afadin-cKO mice died within 3 days after birth, indicating that the afadin-cKO mice developed more severe hydrocephalus than the hyh mutant mice by another mechanism in addition to the denudation of ependymal cells. The gene discussed is AFDN; the disease is Hydrocephalus.