Proteins involved in various stages of cellular differentiation pathways including proteoglycans in cancer, regulation of actin cytoskeleton, focal adhesion, tight junction, ribosome, oxidative phosphorylation, ECM-receptor interaction, and various important signaling pathways including the MAPK signaling, Wnt signaling, Hippo signaling, TGF-beta signaling, PI3K-Akt signaling and calcium signaling pathways were represented by unigenes derived from our EST dataset. The gene discussed is AKT1; the disease is cancer.