Although an increase in PKCδ/θ phosphorylation was observed in non-CF cells following treatment with E2, rottlerin did not prevent E2-induced ASLh decrease in these cells, showing that this kinase is either not involved in the modulation of ion transport by E2 in non-CF cells or that E2 activates alternative signalling pathways that could compensate for the effect of PKCδ activation on ASLh. This evidence concerns the gene PRKCD and cystic fibrosis.