The FH1-FH2 domain and FH2 domain only FMNL3 clones were kindly gifted by Prof. Henry Higgs (New Hampshire, US) transfected into SK-N-AS neuroblastoma cells and filopodia formation was assessed by immunofluorescent staining of the actin cytoskeleton. The gene discussed is FMNL3; the disease is neuroblastoma.