To confirm the specificity of NgBR siRNA, we used a second siRNA (siNgBR2) targeting the coding region of NgBR to confirm both siNgBR1 and siNgBR2 can efficiently knock down NgBR and specifically abolished the estradiol-induced expression of survivin in MCF-7 breast tumor cells (Fig. S2). The gene discussed is NUS1; the disease is breast neoplasm.