However, in HCCC-9810 cells cotransfected with miR-31 inhibitor and RASA1 siRNA, the cell proliferation rate was not downregulated, and instead showed a marginal increase when compared with that in the control group, further indicating that RASA1 acted as a downstream effector of miR-31 and was important in the regulation of ICC cell proliferation. This evidence concerns the gene RASA1 and intrahepatic cholangiocarcinoma.