This could also explain the occurrence of switch in gene expression status from positive in PT to negative in CTC-EBF in case of TWIST1, SNAI1 and SNAI2. Nevertheless, elevated expression of VIM, but not TWIST1 and SNAI1, was observed in TGF-β-treated, motile breast cancer cells in the study of Giampieri et al. [46]. This evidence concerns the gene SNAI2 and breast cancer.