In a previous study on CRC (UICC stages I–IV) [20] B-Raf and CTNNB1 mutations were successfully detected by single strand conformation polymorphism (SSCP) analysis, K-Ras mutations by restriction fragment length polymorphism (RFLP) analysis and APC mutations by direct sequencing of the mutation cluster region. The gene discussed is KRAS; the disease is colorectal carcinoma.