To shed light on the role of the CYP2E1-mediated ROS generation in breast cancer [14,15] the levels of the autophagic markers beclin-1, LC3, Atg5 and Atg7 were monitored in MCF7 and MDA-MB-231 breast cancer cells transiently transfected with CYP2E1 expression vector or shRNA to silence the CYP2E1 expression. This evidence concerns the gene ATG5 and breast cancer.