The pooled HR of 1.34 (95% CI, 1.21-1.48) revealed significant association with overall survival and the Forest plot for this analysis is shown in Figure 4C. Restricting analysis to the twenty studies assessing p53 expression in ESCC gave a pooled HR of 1.26 (95% CI, 1.11-1.42), with ELISA-based studies giving a larger pooled HR (2.13, 95% CI, 1.46-3.09) than IHC-based studies (HR, 1.25, 95% CI, 1.12-1.40). Here, TP53 is linked to esophageal squamous cell carcinoma.