In this study, multivariate analysis (including age, Gleason score, T stage, seminal vesicle invasion, or pre-operative PSA and Gal-3 staining) demonstrated nuclear and cytoplasmic localization of Gal-3 in benign, adjacent-benign and tumor tissues with a significant decrease of its expression from benign to adjacent-benign, and to tumor tissues. This evidence concerns the gene KLK3 and neoplasm.